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KMID : 0606320010170010071
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Journal of Phamacetical Sciences Sookmyung Women s University
2001 Volume.17 No. 1 p.71 ~ p.79
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Screening of Inducible Nitric Oxide Synthase Inhibitory Activity from Herbal Drugs
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Ryu Jae-Ha
Lee So-Young
Park Jae-Hyun
Lee Hwa-Jin
Jang Se-Ran
Eun Jin-Hee
Kim Nam-Yi
Jeong Yeon-Su
Jang Mi-Kyung
Choi Young-Eun
Lee Sook-Hyun
Son Haeng-Ja
Ahn Han-na
Koh Hye-Jin
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Abstract
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Nitric Oxide (NO), derived from L- arginine, is produced by two (constitutive and inducible) of nitric oxide synthase (NOS: CNOS and INOS). The NO produced in large amounts by the INOS is known to be responsible for the vasodilation and hypotension observed in septic shock and inflammation. The inhibitors of INOS, thus, may be useful candidate for the treatment of inflammatory diseases accompanied by the overproduction of NO. We prepared alcoholic extracts of herbal drugs which have been used for the treatment of inflammation in oriental medicine. We have screeaed the inhibitory activity of NO production in lipopolysaccharide (LPS)-activated macrophages after the treatment of these extracts. Among the 81 kinds of extracts of herbal drugs, 34 extracts showed potent inhibitory activity of NO production above 50% at the oncentration of 50 mcg/nil. The inhibitory activities of NO production were also evaluated for several solvent fractions at three different concentrations. specially, hexane soluble fractions of Agrimonia pilosa, Hydrangea serrata, Machilus thunbergii, Prunella vulgaris, Saussurea lappa, Tussilago farfara, and ethyl acetate soluble fractions of Angelica gigas, Ostericum koreanum, Torilis japonica, Perilla frutescence showed moderate activity at 10 and/or 25 mcg/ml. These fractions are promising candidates for the study of the activity-guided chromatographic purification of active compounds.
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